mesenchymal stem cell growth kit for bmsc  (ATCC)

Journal: Biology

Article Title: Grape Pomace Polyphenolic Extract Promotes Osteogenic Differentiation in Human Mesenchymal Stem Cells Through Activation of RUNX2 and NRF2 Transcription Factors: A Potential Natural Strategy for Osteoporosis Prevention

doi: 10.3390/biology15090719

Figure Lengend Snippet: Effects of GPE on human mesenchymal stem viability. BMSC and AdMSC were exposed at increasing concentrations of GPE (1, 5, 10 and 25 µg GAE/mL) for 24 h and cell viability was evaluated by MTT assay. Data are shown as mean ± SD ( n = 5); * p < 0.05 versus untreated control cells (CTR).

Article Snippet: Human bone marrow-derived mesenchymal stem cells (BMSCs) from healthy donors (ATCC-PCS-500-012) were purchased from ATCC (Milan, Italy) as well as mesenchymal stem cell basal medium (ATCC PCS500030) and mesenchymal stem cell growth kit for BMSC (ATCC PCS500041).

Techniques: MTT Assay, Control

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Bone Mesenchymal Stem Cells Promote Extracellular Matrix Remodeling of Degenerated Nucleus Pulposus Cells via the miR-101-3p/EIF4G2 Axis

doi: 10.3389/fbioe.2021.642502

Figure Lengend Snippet: Bone mesenchymal stem cells (BMSCs) promoted ECM remodeling of NPCs. NPCs of patients with IDD were cocultured with BMSCs, with NPCs cultured alone as the control. (A) miR-101-3p, Col-I, and Col-II expressions were detected using the RT-qPCR; (B) MMP-13, Col-I, Col-II, and Aggrecan levels were detected using Western blotting; (C,D) the effects of BMSCs on the viability and apoptosis of NPCs were detected using MTT assay and flow cytometry. The cell experiments were repeated three times independently. Data are presented as mean ± SD. Data in panels (A–C) were analyzed using the independent sample t -test, *** p < 0.001.

Article Snippet: Bone mesenchymal stem cells (BMSCs) and 293T cells were purchased from American Type Culture Collection (Manassas, VA, United States) and cultured in Dulbecco’s modified Eagle’s medium containing 10% of FBS and 100 U/ml of penicillin-streptomycin at 37°C with 5% of CO 2 .

Techniques: Cell Culture, Control, Quantitative RT-PCR, Western Blot, MTT Assay, Flow Cytometry

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Bone Mesenchymal Stem Cells Promote Extracellular Matrix Remodeling of Degenerated Nucleus Pulposus Cells via the miR-101-3p/EIF4G2 Axis

doi: 10.3389/fbioe.2021.642502

Figure Lengend Snippet: Downregulation of miR-101-3p inhibited the effect of BMSCs on ECM remodeling of NPCs. NPCs of patients with IDD were cocultured with BMSCs and then transfected with miR-101-3p inhibitor, with transfection of inhibitor negative control as control. (A) miR-101-3p expressions were detected using the RT-qPCR; (B) MMP-13, Col-I, Col-II, and Aggrecan levels were detected using Western blotting; and (C,D) viability and apoptosis of NPCs were detected using MTT assay and flow cytometry. The cell experiments were repeated three times independently. Data are presented as mean ± SD. Data were analyzed using one-way ANOVA, followed by the Tukey’s multiple comparison test, *** p < 0.001.

Article Snippet: Bone mesenchymal stem cells (BMSCs) and 293T cells were purchased from American Type Culture Collection (Manassas, VA, United States) and cultured in Dulbecco’s modified Eagle’s medium containing 10% of FBS and 100 U/ml of penicillin-streptomycin at 37°C with 5% of CO 2 .

Techniques: Transfection, Negative Control, Control, Quantitative RT-PCR, Western Blot, MTT Assay, Flow Cytometry, Comparison